yuxO [2019-07-10 09:11:24]

similar to thioesterase

locus: BSU31670

pI: 6.47

mw: 13.64 kDa

protein length: 126 aa

gene length: 381 bp

function: unknown

product: unknown

essential: no

synonyms: srfB

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 3,252,405 3,252,785

The protein

Protein family

• UPF0152 (paaI) family (according to Swiss-Prot)

Structure

• [PDB|4QD8] (from Pseudomonas aeruginosa, 45% identity) [pubmed|]

Expression and Regulation

Operons

• genes: [gene|FDACEF9DDC3BEA4EB013AE67BC47ADA532F00205|comQ]-[gene|60D6EB02923D9ADE88F61A8CBBD882BA8BDD457E|comX]-[gene|5A00E233F0B5A0DAB18D2ECC55527F1911987F86|comP]-[gene|7832489F11F606BCF637EC23BAAB41AD10237EBB|comA]-[gene|015989AAB862E418C944AB35DA3605863F059CE9|yuxO]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2507523], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• MGNA-B560 (yuxO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1559 NBRP B. subtilis, Japan]
• BKE31670 ([gene|015989AAB862E418C944AB35DA3605863F059CE9|yuxO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCCATCTCTACACCCCCCA, downstream forward: _UP4_TAAAAAAACAGCCGGAACTC
• BKK31670 ([gene|015989AAB862E418C944AB35DA3605863F059CE9|yuxO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCCATCTCTACACCCCCCA, downstream forward: _UP4_TAAAAAAACAGCCGGAACTC

References

• 2507523